Distance measurement |
To measure cell substrate distance, the cell is attached to a silicon chip with microscopic steps made of transparent silicon oxide. These steps modulate the distance to the silicon chip and thus the fluorescence intensity. The cell substrate distance provides a shift of the fluorescence intensity versus oxide thickness. You can simulate a FLIC distance measurement with the applet below, just move the sliders to change the main parameters. |
[when the applet doesn't work correctly...] |
See the applet with 16 oxides. |